Colony is a naturalistic drama about a family that's torn by opposing forces and makes difficult decisions as its members balance staying together with surviving the struggle of the human race. Set in the near future, the story takes place in Los Angeles, which exists in a state of occupation by a force of outside intruders. Some people choose to collaborate with the authorities and benefit from the new order, while others rebel and suffer the consequences...

The series revolves around the titular room and some of the everyday items from that room which possess unusual powers. The show's protagonist, Joe Miller, is searching for these objects to rescue his daughter, Anna, who has disappeared inside the Room. Once a typical room at a 1960s motel along U.S. Route 66, the Lost Room exists outside of normal time and space since 1961, when what is only referred to as "the Event" took place.