After the events that took place in THE AVENGERS, Agent Phil Coulson (Clark Gregg) returns to the worldwide law enforcement organization S.H.I.E.L.D. where he puts together a small, highly trained, team of Agents to tackle the cases that haven’t been classified yet, the new, the strange and the unknown. That team consists of straight arrow Agent Grant Ward (Brett Dalton), an expert in combat and espionage; pilot and martial artist Agent Melinda May (Ming-Na Wen); and brilliant if socially awkward scientists Agent Leo Fitz (Iain De Caestecker) and Agent Jemma Simmons (Elizabeth Henstridge). They’ll be joined by civilian new recruit and computer hacker Skye (Chloe Bennet).

The Previously unknown purpose of the "Ninth Chevron" is revealed, and ends up taking a team to an Ancient ship "Destiny", a ship built millions of years ago by the Ancients, used to seed Distant galaxies with Stargates. This team, led by Dr. Nicolas Rush and Colonel Everett Young, is trapped on the ship, unable to change its programmed mission. The team encounters new races, new technology and new enemies as the runaway ship travels to the far ends of the Universe.